Composite hydrogels based on modified chitosan with haemostatic and antimicrobial activity
Topic(s) : Industrial applications
Co-authors :
Zoi TERZOPOULOU (GREECE), Ioanna KOUMENTAKOU , Rizos BIKIARISAbstract :
Chitosan (CS), a naturally derived polymer, has emerged as a pivotal component in the development of innovative haemostatic products. Possessing cationic polysaccharide characteristics, CS exhibits bactericidal properties, is renewable, nontoxic, biodegradable, and hydrophilic, thereby promoting coagulation, flocculation, and biosorption. The haemostatic attributes of CS arise from direct electrostatic interactions between negatively charged red blood cells and platelets and the positively charged CS. As a result, researchers and pharmaceutical companies are actively exploring formulations harnessing the haemostatic potential of CS.
This research focuses on advancing haemostatic CS-based materials by incorporating enhanced antimicrobial properties, excellent biocompatibility, rapid haemostatic capabilities, and cost-effectiveness. A grafted chitosan copolymer with [2-(N-morpholino) ethanesulfonic acid (MES)] was synthesized through free-radical polymerization, leading to the creation of CS-g-MES porous sponges via a modified thermal-induced phase separation process.
To further augment the haemostatic potential, the active substance heparin was encapsulated within poly(butylene) succinate (PBSu) nanoparticles, designed to release as an antithrombotic drug after the action of the haemostatic patches. PBSu porous nanoparticles were prepared using dichloromethane (DCM) as a solvent and polyvinyl alcohol (PVA) as a surfactant. DLS and SEM techniques were employed to study the composition, size, and morphology of the nanoparticles, revealing that the size increased from 500 nm for empty nanoparticles to approximately 800 nm when loaded with heparin. FTIR and DSC methods validated the successful synthesis and thermal stability of the produced nanoparticles.
The culmination of these advancements led to the preparation of a chitosan-based composite patch incorporating these nanoparticles, endowed with both haemostatic and improved antimicrobial properties. The composite material was studied through FT-IR, XRD analysis of crystallinity, and SEM imaging. Biological studies further substantiated the antibacterial properties and haemostatic activity of the chitosan dressing, highlighting its potential as an effective and multifunctional wound care solution.
Acknowledgement
Τhe authors wish to acknowledge co-funding of this research by European Union-European Regional Development Fund and Greek Ministry of Education, Research and Religions/EYDE-ETAK through program EPANEK 2014- 2020/Action “EREVNO-DIMIOURGO-KAINOTOMO” (project Τ2ΕΔΚ-00275).
This research focuses on advancing haemostatic CS-based materials by incorporating enhanced antimicrobial properties, excellent biocompatibility, rapid haemostatic capabilities, and cost-effectiveness. A grafted chitosan copolymer with [2-(N-morpholino) ethanesulfonic acid (MES)] was synthesized through free-radical polymerization, leading to the creation of CS-g-MES porous sponges via a modified thermal-induced phase separation process.
To further augment the haemostatic potential, the active substance heparin was encapsulated within poly(butylene) succinate (PBSu) nanoparticles, designed to release as an antithrombotic drug after the action of the haemostatic patches. PBSu porous nanoparticles were prepared using dichloromethane (DCM) as a solvent and polyvinyl alcohol (PVA) as a surfactant. DLS and SEM techniques were employed to study the composition, size, and morphology of the nanoparticles, revealing that the size increased from 500 nm for empty nanoparticles to approximately 800 nm when loaded with heparin. FTIR and DSC methods validated the successful synthesis and thermal stability of the produced nanoparticles.
The culmination of these advancements led to the preparation of a chitosan-based composite patch incorporating these nanoparticles, endowed with both haemostatic and improved antimicrobial properties. The composite material was studied through FT-IR, XRD analysis of crystallinity, and SEM imaging. Biological studies further substantiated the antibacterial properties and haemostatic activity of the chitosan dressing, highlighting its potential as an effective and multifunctional wound care solution.
Acknowledgement
Τhe authors wish to acknowledge co-funding of this research by European Union-European Regional Development Fund and Greek Ministry of Education, Research and Religions/EYDE-ETAK through program EPANEK 2014- 2020/Action “EREVNO-DIMIOURGO-KAINOTOMO” (project Τ2ΕΔΚ-00275).